全文获取类型
收费全文 | 924篇 |
免费 | 86篇 |
出版年
2021年 | 8篇 |
2018年 | 12篇 |
2017年 | 12篇 |
2016年 | 13篇 |
2015年 | 22篇 |
2014年 | 25篇 |
2013年 | 33篇 |
2012年 | 55篇 |
2011年 | 40篇 |
2010年 | 24篇 |
2009年 | 18篇 |
2008年 | 35篇 |
2007年 | 30篇 |
2006年 | 27篇 |
2005年 | 24篇 |
2004年 | 34篇 |
2003年 | 34篇 |
2002年 | 38篇 |
2001年 | 27篇 |
2000年 | 25篇 |
1999年 | 23篇 |
1998年 | 17篇 |
1997年 | 10篇 |
1996年 | 12篇 |
1995年 | 13篇 |
1994年 | 12篇 |
1993年 | 8篇 |
1992年 | 16篇 |
1991年 | 18篇 |
1990年 | 16篇 |
1989年 | 18篇 |
1988年 | 17篇 |
1987年 | 14篇 |
1986年 | 18篇 |
1985年 | 26篇 |
1984年 | 11篇 |
1983年 | 8篇 |
1982年 | 9篇 |
1981年 | 9篇 |
1980年 | 10篇 |
1979年 | 7篇 |
1977年 | 11篇 |
1975年 | 8篇 |
1973年 | 13篇 |
1972年 | 10篇 |
1971年 | 16篇 |
1970年 | 19篇 |
1969年 | 7篇 |
1967年 | 7篇 |
1966年 | 9篇 |
排序方式: 共有1010条查询结果,搜索用时 15 毫秒
61.
The linkage between the polysaccharide and mucopeptide components of the cell wall of Lactobacillus casei 总被引:14,自引:8,他引:6 下载免费PDF全文
1. The linkage between the polysaccharide and mucopeptide components of the cell wall of Lactobacillus casei is rapidly hydrolysed under mild acid-hydrolysis conditions. 2. The release of the polysaccharide is accompanied by the hydrolysis of an N-acetylhexosaminide linkage. The N-acetylhexosamine residue readily forms chromogen and it is concluded that it is substituted on C(3) by the adjacent sugar. 3. Continued heating of the polysaccharide in acid results in a slower release of reactive N-acetylhexosamine due to the hydrolysis of glycosidic linkages within the polysaccharide. 4. After the linkage between the polysaccharide and mucopeptide has been hydrolysed, acid phosphatase will release approx. 40% of the total phosphorus as inorganic phosphate. 5. It is concluded that the polysaccharide component of the cell wall is joined through its reducing end group to a phosphate grouping in the mucopeptide. 相似文献
62.
63.
Summary Gibberellic-acid(GA3)-induced -amylase has been localised in barley aleurone layers using cytochemical methods and light microscopy. Evidence obtained from the use of a starch substrate film method as well as immunofluorescence indicated that the first amylase to appear in the cell was associated with aleurone grains, apparently with the outer membrane, and also with the peripheral cytoplasm. In GA3-treated tissue, the amylase distribution was much more diffuse, although patchy, throughout the cytoplasm and it tended to accumulate in the endosperm side of the cell. The possibility that the aleurone grain membrane is the site of gibberellin-induced enzyme synthesis and that it proliferates to become rough endoplasmic reticulum is considered. Immunological information was obtained which supports earlier indications that induced -amylase consists of two different proteins, each with molecular heterogeneity. 相似文献
64.
The role of sulfhydryl groups in the activity of type A botulinum toxin 总被引:10,自引:0,他引:10
65.
66.
CAMILLA S. ALMEIDA PAULO F. CRISTALDO OG DESOUZA LEANDRO BACCI DANIELA F. FLORENCIO NAYARA G. CRUZ ABRAÃO A. SANTOS ALISSON S. SANTANA ALEXANDRE P. OLIVEIRA ANA P. S. LIMA ANA P. A. ARAÚJO 《Ecological Entomology》2018,43(3):371-378
1. Resource density can regulate the area that animals use. At low resource density, there is a conflict in terms of balance between costs of foraging and benefits acquired. The foraging of the higher termite Nasutitermes aff. coxipoensis consists of searching throughout trails and a building galleries phase. 2. In this study, a manipulative field experiment was used to test the hypothesis that colonies of N. aff. coxipoensis forage towards a more profitable balance between the establishment of trails and gallery construction at low resource density. 3. The experiment was conducted in north‐eastern Brazil. Seven experimental plots were established with a continuous increase in resource density (sugarcane baits). Entire colonies of N. aff. coxipoensis were transplanted from their original sites to the experimental plot, totalling 35 nests. The number, branches and total length of trails and galleries were quantified. 4. The results show that N. aff. coxipoensis optimises its foraging output, intensifying the establishment of trails at the cost of gallery construction when resource density is low. The number of trails, the number of trail branches and the total length of trails decreased with increasing resource density. Interestingly, at low resource density, the search effort was concentrated on forming longer and a greater number of trails, a small proportion of which were converted into galleries. The opposite relationship was observed at high resource density. 5. These results suggest an optimisation of search efforts during foraging depending on resource density, a mechanism that may help researchers to understand the use of space by higher termite species. 相似文献
67.
Rosie A. Fisher Charles D. Koven William R. L. Anderegg Bradley O. Christoffersen Michael C. Dietze Caroline E. Farrior Jennifer A. Holm George C. Hurtt Ryan G. Knox Peter J. Lawrence Jeremy W. Lichstein Marcos Longo Ashley M. Matheny David Medvigy Helene C. Muller‐Landau Thomas L. Powell Shawn P. Serbin Hisashi Sato Jacquelyn K. Shuman Benjamin Smith Anna T. Trugman Toni Viskari Hans Verbeeck Ensheng Weng Chonggang Xu Xiangtao Xu Tao Zhang Paul R. Moorcroft 《Global Change Biology》2018,24(1):35-54
Numerous current efforts seek to improve the representation of ecosystem ecology and vegetation demographic processes within Earth System Models (ESMs). These developments are widely viewed as an important step in developing greater realism in predictions of future ecosystem states and fluxes. Increased realism, however, leads to increased model complexity, with new features raising a suite of ecological questions that require empirical constraints. Here, we review the developments that permit the representation of plant demographics in ESMs, and identify issues raised by these developments that highlight important gaps in ecological understanding. These issues inevitably translate into uncertainty in model projections but also allow models to be applied to new processes and questions concerning the dynamics of real‐world ecosystems. We argue that stronger and more innovative connections to data, across the range of scales considered, are required to address these gaps in understanding. The development of first‐generation land surface models as a unifying framework for ecophysiological understanding stimulated much research into plant physiological traits and gas exchange. Constraining predictions at ecologically relevant spatial and temporal scales will require a similar investment of effort and intensified inter‐disciplinary communication. 相似文献
68.
69.
Shelley W. Peterson Natalie C. Knox George R. Golding Shaun D. Tyler Andrea D. Tyler Philip Mabon Joanne E. Embree Fiona Fleming Sergio Fanella Gary Van Domselaar Michael R. Mulvey Morag R. Graham 《PloS one》2016,11(3)
Whereas the infant gut microbiome is the subject of intense study, relatively little is known regarding the nares microbiome in newborns and during early life. This study aimed to survey the typical composition and diversity of human anterior nare microflora for developing infants over time, and to explore how these correlate to their primary caregivers. Single nare swabs were collected at five time points over a one-year period for each subject from infant-caregiver pairs. Our study comprised of 50 infants (recruited at 2 weeks, post delivery) and their 50 primary caregivers. Applying the chaperonin-60 (cpn60) universal target (UT) amplicon as our molecular barcoding marker to census survey the microbial communities, we longitudinally surveyed infant nares microbiota at 5 time points over the course of the first year of life. The inter- and intra-subject diversity was catalogued and compared, both longitudinally and relative to their adult primary caregivers. Although within-subject variability over time and inter-subject variability were both observed, the assessment detected only one or two predominant genera for individual infant samples, belonging mainly to phyla Actinobacteria, Firmicutes, and Proteobacteria. Consistent with previously observed microbial population dynamics in other body sites, the diversity of nares microflora increased over the first year of life and infants showed differential operational taxonomic units (OTUs) relative to their matched primary caregiver. The collected evidence also support that both temporal and seasonal changes occur with respect to carriage of potentially pathogenic bacteria (PPBs), which may influence host predisposition to infection. This pilot study surveying paired infant/caregiver nare microbiomes provides novel longitudinal diversity information that is pertinent to better understanding nare microbiome development in infants. 相似文献
70.
M. D. Furones † D. J. Alderman D. Bucke T. C. Fletcher ‡§ D. Knox ‡ A. White ‡§ 《Journal of fish biology》1992,41(6):1037-1041
Rainbow trout fed diets containing 7, 86 or 806 mg vitamin E kg−1 for 22 weeks were exposed to virulent Yersinia ruckeri by bath or injection. Mortalities were always least among those fed the highest concentration of vitamin E but serum antibody production was not affected by vitamin E levels. 相似文献